Author: Chow, Ken Yan Ching; Hon, Chung Chau; Hui, Raymond Kin Hi; Wong, Raymond Tsz Yeung; Yip, Chi Wai; Zeng, Fanya; Leung, Frederick Chi Ching
Title: Molecular Advances in Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) Document date: 2016_11_28
ID: xuj4yymz_5
Snippet: The etiological entity of a viral infection relies on both molecular and traditional virological methods includ-ing serological techniques, virus isolation by cell culture, and electron microscopy (2 , 10 ) . Both molecular approaches and conventional approaches were employed for the initial characterization of the SARS pathogen (2 ) . Peiris et al (2 ) firstly isolated the virus from in vitro tissue culture and subsequently yielded a 646-bp geno.....
Document: The etiological entity of a viral infection relies on both molecular and traditional virological methods includ-ing serological techniques, virus isolation by cell culture, and electron microscopy (2 , 10 ) . Both molecular approaches and conventional approaches were employed for the initial characterization of the SARS pathogen (2 ) . Peiris et al (2 ) firstly isolated the virus from in vitro tissue culture and subsequently yielded a 646-bp genomic fragment by RT-PCR using degenerate primers, which showed more than 50% homology to the RNA polymerase gene of bovine coronavirus (BCV) and murine hepatitis virus (MHV). The use of gene chip further confirmed the coronavirus as a possible cause of SARS (11 ) .
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