Author: Shefali Dobhal; Gamze Boluk; Brooke Babler; Michael J. Stulberg; John Rascoe; Mark Nakhla; Toni A. Chapman; Alex B. Crockford; Michael Melzer; Anne M. Alvarez; Mohammad Arif
Title: Comparative genomics reveals signature regions used to develop a robust and sensitive multiplex TaqMan real-time qPCR assay to detect the genus Dickeya and Dickeya dianthicola Document date: 2019_11_20
ID: lgeu4id0_27
Snippet: The primer sets with and without flap sequences were compared for their sensitivity using the purified D. dianthicola genomic DNA. Both primer sets with and without flaps were sensitive and detected 10 fg of purified genomic DNA ( Figure 5A1, A2; 5B1, B2 ). The primers with the 5' ATrich flap showed better reaction efficiency compared to the primer set with no flap (Figure 5A1 , A2). At lower concentrations of template DNA, the primers with no AT.....
Document: The primer sets with and without flap sequences were compared for their sensitivity using the purified D. dianthicola genomic DNA. Both primer sets with and without flaps were sensitive and detected 10 fg of purified genomic DNA ( Figure 5A1, A2; 5B1, B2 ). The primers with the 5' ATrich flap showed better reaction efficiency compared to the primer set with no flap (Figure 5A1 , A2). At lower concentrations of template DNA, the primers with no AT-rich flap sequences showed higher standard deviation among the replicates compared to primers with flap, indicating the enhanced stability and better reaction thermodynamics.
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