Title: Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017 Document date: 2017_11_7
ID: roslkxeq_222
Snippet: Blood was obtained from healthy adult Walker hounds before and after immunosuppressive treatment at standard doses using a randomized crossover study design. Treatments included azathioprine, cyclosporine, mycophenolate mofetil, leflunomide, or prednisone. Blood was sampled prior to and following 7 days of treatment. Four dogs were tested for each drug with a minimum wash out period of three weeks. Whole blood was activated with either phorbol 12.....
Document: Blood was obtained from healthy adult Walker hounds before and after immunosuppressive treatment at standard doses using a randomized crossover study design. Treatments included azathioprine, cyclosporine, mycophenolate mofetil, leflunomide, or prednisone. Blood was sampled prior to and following 7 days of treatment. Four dogs were tested for each drug with a minimum wash out period of three weeks. Whole blood was activated with either phorbol 12-myristate 13-1 acetate and ionomycin (PMA/I) or lipopolysaccharide (LPS). Cytokines in the supernatant were analyzed using a multiplex assay. The Kruskal-Wallis test was used to test for carryover effects of the drugs. The Wilcoxon signed-rank test was used to assess each activator effect on cytokine production.
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