Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer Document date: 2020_1_1
ID: tymoeyoo_61
Snippet: For mAb cleavage by recombinant human trypsin/PRSS1, recombinant PRSS1 and mAbs were combined in 1 ml of PBS and then incubated in a humidified atmosphere at 37°C. The recombinant PRSS1-mAb intermixture (200 ïl) was collected at 0, 2, 6, 12, 24, 36, and 48 hours. For mAb cleavage by CRC cell-secreted PRSS1, 4 ml of serum-free medium harboring the mAbs was added to culture plates containing 80 to 90% cells, which were then incubated in a 5% CO .....
Document: For mAb cleavage by recombinant human trypsin/PRSS1, recombinant PRSS1 and mAbs were combined in 1 ml of PBS and then incubated in a humidified atmosphere at 37°C. The recombinant PRSS1-mAb intermixture (200 ïl) was collected at 0, 2, 6, 12, 24, 36, and 48 hours. For mAb cleavage by CRC cell-secreted PRSS1, 4 ml of serum-free medium harboring the mAbs was added to culture plates containing 80 to 90% cells, which were then incubated in a 5% CO 2 humidified atmosphere at 37°C. The supernatant (500 ïl) was collected at 0, 2, 6, 12, 24, 36, and 48 hours. We also collected the cell culture supernatant of CRC cells cultured for 24 hours without serum, added the mAbs to this supernatant, and incubated the mixture in a humidified atmosphere at 37°C for 24 or 48 hours.
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