Selected article for: "Coomassie Blue staining and expression vector"
Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer
  • Document date: 2020_1_1
    • ID: tymoeyoo_70
    Snippet: Human SPINK1 coding regions were PCR-amplified from a human mammary retroviral complementary DNA library and cloned into the prokaryotic expression vector pET22b. The primers used were as follows: forward, 5′-GAATTCTAATGGACTCCCTGGGAAGA-GAGGCC-3′; reverse, 5′-CTCGAGGCAAGGCCCAGATTTTTGAA-3′. The fusion protein SPINK1 was expressed in Escherichia coli and purified by nickel affinity and size exclusion chromatography. Isopropyl--d-thiogalac.....
    Document: Human SPINK1 coding regions were PCR-amplified from a human mammary retroviral complementary DNA library and cloned into the prokaryotic expression vector pET22b. The primers used were as follows: forward, 5′-GAATTCTAATGGACTCCCTGGGAAGA-GAGGCC-3′; reverse, 5′-CTCGAGGCAAGGCCCAGATTTTTGAA-3′. The fusion protein SPINK1 was expressed in Escherichia coli and purified by nickel affinity and size exclusion chromatography. Isopropyl--d-thiogalactopyranoside (0.2 mM/liter) induction at 16°C for 16 hours was estimated to be an optimal expression strategy. The expression of the fusion proteins was detected by Western blotting and Coomassie Brilliant Blue G-250 staining.

    Search related documents:
    Co phrase search for related documents
    • Escherichia coli and size exclusion: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • Escherichia coli and size exclusion chromatography: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • Escherichia coli and size exclusion chromatography nickel affinity: 1
    • Escherichia coli and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • expression strategy and western blotting: 1, 2, 3, 4
    • expression vector and size exclusion: 1, 2, 3, 4, 5
    • expression vector and size exclusion chromatography: 1, 2, 3, 4, 5
    • expression vector and size exclusion chromatography nickel affinity: 1
    • expression vector and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
    • fusion protein and size exclusion: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • fusion protein and size exclusion chromatography: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • fusion protein and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • fusion protein expression and size exclusion: 1
    • fusion protein expression and size exclusion chromatography: 1
    • fusion protein expression and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • mm liter and size exclusion: 1
    • mm liter and size exclusion chromatography: 1
    • nickel affinity and size exclusion chromatography nickel affinity: 1, 2, 3, 4
    • nickel affinity and western blotting: 1, 2