Author: Rogers, J.; Schoepp, R.J.; Schröder, O.; Clements, T.L.; Holland, T.F.; Li, J.Q.; Li, J.; Lewis, L.M.; Dirmeier, R.P.; Frey, G.J.; Tan, X.; Wong, K.; Woodnutt, G.; Keller, M.; Reed, D.S.; Kimmel, B.E.; Tozer, E.C.
Title: Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases Document date: 2008_5_13
ID: xkx56h0o_19
Snippet: Supernatants were harvested following a 25 min spin at 2000 g. The antibodies were purified from the supernatants using Protein G HP columns (Amersham, Piscataway, NJ, USA) by fast protein liquid chromatography (FPLC). The purified IgGs were quantified in BCA protein assays, analyzed on SDS gels, and assayed for the presence of endotoxin using a chromogenic Limulus Amebocyte Lysate test (Lonza, Rockland, ME, USA). If necessary, the preparations w.....
Document: Supernatants were harvested following a 25 min spin at 2000 g. The antibodies were purified from the supernatants using Protein G HP columns (Amersham, Piscataway, NJ, USA) by fast protein liquid chromatography (FPLC). The purified IgGs were quantified in BCA protein assays, analyzed on SDS gels, and assayed for the presence of endotoxin using a chromogenic Limulus Amebocyte Lysate test (Lonza, Rockland, ME, USA). If necessary, the preparations were passed through polymyxin B gel columns (Detoxi-Gel, Pierce 20344) for endotoxin removal.
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