Title: Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017 Document date: 2017_11_7
ID: roslkxeq_333
Snippet: Total RNA was purified from frozen tissue and treated with DNase. One-step RT-PCR assays amplifying FcaGHV1 ORF73, F7, and ORF50 (mRNA splice product) were designed and optimized. The identity of RT-PCR products migrating at the expected size was confirmed by sequencing. In 5 cases, expression of 1, 2 or 3 FcaGHV1 genes was detected. Four cats tested negative for all three transcripts......
Document: Total RNA was purified from frozen tissue and treated with DNase. One-step RT-PCR assays amplifying FcaGHV1 ORF73, F7, and ORF50 (mRNA splice product) were designed and optimized. The identity of RT-PCR products migrating at the expected size was confirmed by sequencing. In 5 cases, expression of 1, 2 or 3 FcaGHV1 genes was detected. Four cats tested negative for all three transcripts.
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