Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017

Disclosures: Disclosures to report. Jacob ME: Director of NCSU Clinical Microbiology Laboratories. No other disclosures to report. Klebsiella pneumoniae are important nosocomial pathogens that are increasingly reported as multidrug-resistant (MDR). Companion animals (CA) with urinary tract infections (UTI) may become infected with MDR and virulent K. pneumoniae and thus act as reservoir to humans. This study aimed to characterize and compare the clonal relatedness of K. pneumoniae isolated from companion animals and humans with UTI. K. pneumoniae isolated from CA (n = 26) and from community and hospital-acquired human UTIs (n = 76) were tested by disk diffusion for susceptibility (AST) against 28 antimicrobials according to CLSI. Resistant isolates were screened for sixteen resistance genes and seven virulence genes by PCR. All CA isolates and third-generation cephalosporin resistant (3GCr) human isolates were typed by MLST. Population structure of CA and human isolates were further characterized by PFGE-XbaI macro-restriction using Dice/UPGMA clustering analysis with a 1.5% tolerance.