Selected article for: "detection sensitivity and RT PCR sensitivity"

Author: Neethirajan, Suresh; Ahmed, Syed Rahin; Chand, Rohit; Buozis, John; Nagy, Éva
Title: Recent Advances in Biosensor Development for Foodborne Virus Detection
  • Document date: 2017_7_5
  • ID: sj6zfybb_102
    Snippet: Several novel biosensors have been developed in the detection of hepatitis B viruses based on impedimetric genosensor, plasmonic biosensor, and electrochemical immunosensor techniques. An impedimetric genosensor is developed using zeolite nanocrystals and multiwalled carbon nanotubes to detect PCR amplified single-stranded virus DNA [109] . The DNA hybridisation is detected as the nanocrystals are placed on a glass electrode constructed from fluo.....
    Document: Several novel biosensors have been developed in the detection of hepatitis B viruses based on impedimetric genosensor, plasmonic biosensor, and electrochemical immunosensor techniques. An impedimetric genosensor is developed using zeolite nanocrystals and multiwalled carbon nanotubes to detect PCR amplified single-stranded virus DNA [109] . The DNA hybridisation is detected as the nanocrystals are placed on a glass electrode constructed from fluorine doped tin oxide, while the nanotubes are incorporated to enhance electro-conductivity. The detection results are compared to RT-PCR detection with 97% accuracy; and the sensitivity of detection limit is 50 copies/mL. The electrode can be reused by dipping in 0.1 M NaOH for three minutes with a loss of its initial activity as 50% in four weeks.

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