Recent Advances in Biosensor Development for Foodborne Virus Detection

Neethirajan, Suresh; Ahmed, Syed Rahin; Chand, Rohit; Buozis, John; Nagy, Éva

In the SELEX process, nitrocellulose membranes, agarose gels, and adsorptive microplates are used as supports to immobilise virus targets, but the subsequent separation under these supports is time-consuming and tedious. To eliminate the carrier defect, a study applies carrier magnetic nanoparticles to immobilise the surface antigen of hepatitis B viruses [131] . DNA aptamers are selected from a library after 13 selection rounds and used to build an aptasensor based on chemiluminescence immunoassay and magnetic separation. The derived aptasensor can be used to detect the virus surface antigen from purified or actual samples with a limit of detection limit as 0.1 ng/mL, which is lower than the limit of 0.5 ng/mL from ELISA measurement.