Purification and characterization of monoclonal IgG antibodies recognizing Ebola virus glycoprotein

Han, Baek-Sang; Jang, Ho-Young; Racine, Trina; Qiu, Xiangguo; Sin, Jeong-Im

For intramuscular (IM)-electroporation (EP) delivery of DNA vaccines, mice were injected intramuscularly with 50 µg of pcDNA3-GP per mouse in a final volume of 50 µL of phosphate-buffered saline (PBS) using a 31-gauge needle. The injections were followed by EP at 0.2 volts for 4 seconds using Cellectra of GeneOne Life Science (Seoul, Korea) in accordance with the manufacturer's protocol. Plasmid DNA was produced in bacteria and purified using endotoxin-free Qiagen kits according to the manufacturer's protocol (Qiagen, Valencia, CA, USA). For delivery of recombinant GP, mice were injected intraperitoneally with 20 µg of GP per mouse emulsified in 400 µL of incomplete Freund's adjuvant. Mice were also injected intravenously with 20 µg of recombinant GP per mouse in a final volume of 200 µL of PBS.