Author: Neethirajan, Suresh; Ahmed, Syed Rahin; Chand, Rohit; Buozis, John; Nagy, Éva
Title: Recent Advances in Biosensor Development for Foodborne Virus Detection Document date: 2017_7_5
ID: sj6zfybb_121
Snippet: Aptamers fit the requirements of foodborne virus POC detection to be fast, accurate, easy to use, and affordable [129] . They provide higher discriminating capacity of serotypes and faster adaptation to continuously evolving virus strains. For example, a DNA aptamer strategy has been introduced to detect murine and human noroviruses; and a promising aptamer has been selected post nine-rounds of SELEX with very high affinity to both virus types [1.....
Document: Aptamers fit the requirements of foodborne virus POC detection to be fast, accurate, easy to use, and affordable [129] . They provide higher discriminating capacity of serotypes and faster adaptation to continuously evolving virus strains. For example, a DNA aptamer strategy has been introduced to detect murine and human noroviruses; and a promising aptamer has been selected post nine-rounds of SELEX with very high affinity to both virus types [130] . The binding affinity of the selected aptamer AG3 is a million-fold higher than a non-specific DNA control sequence, and has a million-fold higher selectivity from a similar virus, feline calicivirus. The aptasensor combining AG3 and biosensor technique using gold nanoparticles can detect norovirus particles with a limit of detection as 180 copies/mL in environmental samples.
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