Selected article for: "derived aptasensor and detection limit"
Author: Neethirajan, Suresh; Ahmed, Syed Rahin; Chand, Rohit; Buozis, John; Nagy, Éva
Title: Recent Advances in Biosensor Development for Foodborne Virus Detection
  • Document date: 2017_7_5
    • ID: sj6zfybb_124
    Snippet: Another aptamer study targets the P domain protein of human GII.4 norovirus strain using E. coli to express and purify the P protein [133] . After SELEX, an aptamer M6-2 is selected and confirmed by ELISA targeting GI.7, GII.2, two GII.4, and GII.7 strains with low to moderate binding affinity. Aptamer magnetic capture and RT-PCR are combined with the selected M6-2 aptamer to capture noroviruses with a detection limit of 4.88 log10 input genomic .....
    Document: Another aptamer study targets the P domain protein of human GII.4 norovirus strain using E. coli to express and purify the P protein [133] . After SELEX, an aptamer M6-2 is selected and confirmed by ELISA targeting GI.7, GII.2, two GII.4, and GII.7 strains with low to moderate binding affinity. Aptamer magnetic capture and RT-PCR are combined with the selected M6-2 aptamer to capture noroviruses with a detection limit of 4.88 log10 input genomic copies. The M6-2 derived aptasensor was successfully tested for its capturing capability of purified GII.4 New Orleans outbreak noroviruses.

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