Author: Neethirajan, Suresh; Ahmed, Syed Rahin; Chand, Rohit; Buozis, John; Nagy, Éva
Title: Recent Advances in Biosensor Development for Foodborne Virus Detection Document date: 2017_7_5
ID: sj6zfybb_129
Snippet: The commonly used enzymes in pathogen detection are alkaline phosphatase, beta-galactosidase, and horseradish peroxidase (HRP). A colorimetric assay based on alkaline phosphatase induced metallisation is applied to the detection of H9N2 avian influenza virus particles linked to immnomagnetic separation [135] . The color change induced when enzymes are combined with gold nanoparticle-induced silver deposition to enhance the optical signals as 4-6 .....
Document: The commonly used enzymes in pathogen detection are alkaline phosphatase, beta-galactosidase, and horseradish peroxidase (HRP). A colorimetric assay based on alkaline phosphatase induced metallisation is applied to the detection of H9N2 avian influenza virus particles linked to immnomagnetic separation [135] . The color change induced when enzymes are combined with gold nanoparticle-induced silver deposition to enhance the optical signals as 4-6 folds higher than similar metal nanoparticle colorimetric methods. It is coupled with a magnetic bead-based sandwich immunoassay to detect the viruses in complex samples, such as chicken serum, without pretreatment and with a limit of detection as 17.5 pg/mL.
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