Selected article for: "complementary dna and mini kit"
Author: Lan, Jiaming; Yao, Yanfeng; Deng, Yao; Chen, Hong; Lu, Guangwen; Wang, Wen; Bao, Linlin; Deng, Wei; Wei, Qiang; Gao, George F.; Qin, Chuan; Tan, Wenjie
Title: Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge()
  • Document date: 2015_8_18
    • ID: q8i2gfut_23
    Snippet: Total RNA was isolated from individual samples using the RNeasy Mini Kit (Qiagen) and reverse transcription reactions were performed using the Superscript III First Strand Synthesis Kit (Invitrogen), as described previously (Yao et al., 2013) , to derive complementary DNA (cDNA). All cDNA samples were stored at −20°C until use in the qRT-PCR, targeted to upE as described previously (Corman et al., 2012) ......
    Document: Total RNA was isolated from individual samples using the RNeasy Mini Kit (Qiagen) and reverse transcription reactions were performed using the Superscript III First Strand Synthesis Kit (Invitrogen), as described previously (Yao et al., 2013) , to derive complementary DNA (cDNA). All cDNA samples were stored at −20°C until use in the qRT-PCR, targeted to upE as described previously (Corman et al., 2012) .

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