Author: Lan, Jiaming; Yao, Yanfeng; Deng, Yao; Chen, Hong; Lu, Guangwen; Wang, Wen; Bao, Linlin; Deng, Wei; Wei, Qiang; Gao, George F.; Qin, Chuan; Tan, Wenjie
Title: Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge() Document date: 2015_8_18
ID: q8i2gfut_19
Snippet: The virus neutralisation assay was conducted on Vero Cells. Briefly, sera were diluted twofold and mixed with 50 μL of 2 × 10 3 TCID 50 /mL virus, incubated at 37°C for 1 h in quadruplicate. Thereafter, 100-μL virus-serum mixture was added to Vero cells previously seeded at 1.5 × 10 4 /well. Inoculated plates were incubated in a CO 2 incubator at 37°C for 3 days, following which the cytopathic effects (CPEs) of the virus were observed micro.....
Document: The virus neutralisation assay was conducted on Vero Cells. Briefly, sera were diluted twofold and mixed with 50 μL of 2 × 10 3 TCID 50 /mL virus, incubated at 37°C for 1 h in quadruplicate. Thereafter, 100-μL virus-serum mixture was added to Vero cells previously seeded at 1.5 × 10 4 /well. Inoculated plates were incubated in a CO 2 incubator at 37°C for 3 days, following which the cytopathic effects (CPEs) of the virus were observed microscopically at 40 × magnification. All tests were repeated twice independently.
Search related documents:
Co phrase search for related documents- cytopathic effect and Vero cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- cytopathic effect and virus neutralisation: 1, 2
- cytopathic effect and virus neutralisation assay: 1
- cytopathic effect and virus serum: 1, 2, 3, 4, 5, 6
- inoculated plate and virus serum: 1
- Vero cell and virus neutralisation: 1
- Vero cell and virus serum: 1, 2, 3, 4, 5, 6, 7, 8
- Vero cell and virus serum mixture: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date