Quantitative determination of residual 1,4-dioxane in three-dimensional printed bone scaffold

Li, Ling; Long, Jing; Li, Long; Cao, Huijuan; Tang, Tingting; Xi, Xinghua; Qin, Ling; Lai, Yuxiao; Wang, Xinluan

All of the standard solutions described above (4 mL) were transferred to each vial and the vials were closed immediately. After heating the vial for 40 minutes at 90 C, the HS-GC-MS of the gas in the sample vial was performed for optimisation of solutions. Quantitation A 0.3 g sample was weighed accurately and cut into pieces of 0.2 Â 0.2 Â 0.2 cm 3 in a headspace vial. DMF (4 mL) was transferred to this vial, and the vial was closed immediately. After heating the vial for 40 minutes at 90 C, the HS-GC-MS of the gas in the sample vial was performed in the selective ion (masses 88) mode. The sample was quantified by the external standard method. Calibration curves were determined at the beginning of sample set in 1 day. Blanks and check standards were run for each sample set. Overall, blanks and check standards comprised a minimum of 30% of the total samples analysed during any given analysis sequence to ensure the stability of the method.