Quantitative determination of residual 1,4-dioxane in three-dimensional printed bone scaffold

Li, Ling; Long, Jing; Li, Long; Cao, Huijuan; Tang, Tingting; Xi, Xinghua; Qin, Ling; Lai, Yuxiao; Wang, Xinluan

In this investigation, two solvents (double distilled H 2 O, DMF) and two sodium solutions (NaCl solution and Na 2 CO 3 solution) were chosen as optimising solvents for sample preparation (Figure 2A ). The order of the peak area of 1,4dioxane was: double distilled Na 2 CO 3 eH 2 O > NaCleH 2 O > H 2 O > DMF (p < 0.01 vs. H 2 O, n Z 3) ( Figure 2B ). However, the area of 1,4-dioxane in each concentration of the calibration curves without matrix were twice that of those in which matrix was dissolved with Na 2 CO 3 eH 2 O. These differences were not found between the calibration curves with or without matrix blank scaffolds dissolved with DMF The retention time of the target mass was 3.266 min. There was 100% chromatographic resolution of the peaks (resolution, (RS) > 1.5) (Figure 3) . The tailing factor of this target peak was 2.26, and the number of theoretical plates was 36,954.