Selected article for: "cytoplasm nucleus and western blot"
Author: Wojciechowska, Marzena; Olejniczak, Marta; Galka-Marciniak, Paulina; Jazurek, Magdalena; Krzyzosiak, Wlodzimierz J.
Title: RAN translation and frameshifting as translational challenges at simple repeats of human neurodegenerative disorders
  • Document date: 2014_10_29
    • ID: utigp2vi_10
    Snippet: RAN translation was shown to depend on the repeat length, and the abundance of nascent proteins tends to increase with the repeat number. In SCA8, polyAla was the most expressed, followed by polyGln and polySer, and the repeat lengths at which robust levels of these proteins were detected by western blot in HEK293T cells were 73, 42 and 58, respectively (Supplementary Table S1 ). These three proteins can be co-expressed in a single cell; however,.....
    Document: RAN translation was shown to depend on the repeat length, and the abundance of nascent proteins tends to increase with the repeat number. In SCA8, polyAla was the most expressed, followed by polyGln and polySer, and the repeat lengths at which robust levels of these proteins were detected by western blot in HEK293T cells were 73, 42 and 58, respectively (Supplementary Table S1 ). These three proteins can be co-expressed in a single cell; however, their levels varied significantly among cells, as shown by immunofluorescence analysis. In addition, the intracellular localization of the RAN translation products varies, and polyAla proteins are dispersed in the cytoplasm but may form aggregates in the nucleus. PolyGln forms nuclear aggregates, and the least abundant polySer forms both cytoplasmic and nuclear aggregates (14) .

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