Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017

Disclosures: No disclosures to report. . Whole transcriptome next-generation sequencing (NGS) was performed on an Illumina NextSeq500 system. Tissue samples were examined histologically: Nine were diagnosed as malignant and nine were classified as non-neoplastic. Cytological examination of US-FNA was possible in 14 cases, eleven being diagnosed as normal to hyperplastic and three specimens being classified as prostate carcinoma. RNA concentration was detectable in all samples ranging from 9 ng/µL to 99 ng/µL. RNA quantity was sufficient as starting material for NGS. Transcriptome analyses from samples with RIN value ≥5.5 were successful. Data sets of differentially expressed genes (DEGs) were summarized in principal component analysis and showed major variances in DEGs between non-neoplastic and malignant samples and minor differences between US-FNA and tissue with comparable diagnosis. Globin genes were identified and significantly upregulated in US-FNA samples. Based on isolated total RNA concentration and integrity, residual cells from diagnostic US-FNA of the canine prostate can be considered as an adequate sample source for gene expression studies, biomarker research and a potential tool for advanced diagnostic of canine prostatic diseases. Disclosures: No disclosures to report. Histiocytic sarcoma (HS) is an aggressive neoplasm of dogs. Overrepresentation has been documented in several breeds, including Bernese mountain dogs, flat-coated retrievers, golden retrievers, and Rottweilers. The purpose of this retrospective study was to describe a series of miniature schnauzers (MS) diagnosed with HS in the UK and assess whether they were over-represented when compared to the hospital population. Medical records of MS with a cytological or histopathological diagnosis of HS between January 2010 and March 2017 were reviewed. Breed predisposition was assessed with odds ratios, using the total number of hospital admissions for each breed without HS admitted during the study period as controls. This was also performed where two or more cases with HS were recorded for a particular breed.