Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017

Disclosures: No disclosures to report. The aim of this prospective study was to investigate bacteremia in puppies with enteritis attributed to canine parvovirus 2 (CPV-2) infection. Blood samples were obtained for culture from 17 puppies with parvoviral enteritis (Group 1), aged 2-10 months, prior to treatment on admission (time 1), 48 hours later (time 2), and on the day of sudden deterioration or discharge (time 3). Thirteen healthy puppies (Group 2), aged 2-6 months, were selected as controls and sampled for blood culture once. Dogs were eligible for inclusion in Group 1 according to clinical and clinicopathological abnormalities, incomplete vaccination against canine parvoviral enteritis, and presence of CPV antigens (rapid immunoassay) and of CPV-2 DNA (real-time PCR) in feces. Group 2 dogs were healthy on physical examination, complete blood count, serum biochemistry, urinalysis, abdominal ultrasonography, buffy coat cytology, and tested negative for CPV antigens and CPV-2 DNA in feces. All animals had not received antimicrobials for 4 weeks prior to inclusion in the study. Blood samples from all dogs were aseptically obtained from both jugular veins at each time-point, placed in pediatric culture bottles and shipped to the laboratory immediately. Blood cultures were performed by BACTEC 9120 blood culture instrument (Becton Dickinson) using pediatrics (PEDS) bottles. Isolates from positive blood cultures were identified to the species level by the VITEK 2 automated system (Biomerieux, France). Standardized treatment included intravenous administration of fluids, maropitant, metoclopramide, ampicillin and enrofloxacin.