Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017

Disclosures: No disclosures to report. The dissemination of ESBL and Carbapenemase producing Klebsiella pneumoniae is a worldwide concern. Multidrug-resistant (MDR) K. pneumoniae UTIs in companion animals raise great concerns regarding their role as reservoirs and in the spread of such bacteria. The aim of this study was to evaluate the withinhousehold sharing of ESBL-producing MDR K. pneumoniae from a UTI infected cat. Antimicrobial susceptibility testing of the uropathogenic bacteria was conducted by microdilution methods according to CLSI and screening for the presence of CTX-M ESBL was performed by PCR and sequencing. Fecal samples from the infected cat (ICat) and co-habiting pets (Cat-1, Dog-1, Dog-2) were collected for detection and quantification of colonization by the infection K. pneumoniae strain. Environment swabs from the animals food/water bowls and bedding were also collected. Samples were serially diluted and cultured in MacConkey agar plates containing 1.5 lg/ mL cefotaxime (CTX). Negative samples were grown in enrichment media prior to plating to detect low levels of colonization. When positive, up to five K. pneumoniae colonies were obtained for further analysis. Unique clones were resolved by PFGE-XbaI macro-restriction using Dice/UPGMA clustering analysis.