Selected article for: "cell supernatant and specific antibody"
Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer
  • Document date: 2020_1_1
    • ID: tymoeyoo_52
    Snippet: The CRC cell lines were plated in six-well plates. After 24 hours, the cells were washed with PBS and cultured for 24 hours in serum-free medium. The cell supernatant and protein were collected for immunoblotting. We also detected the biochemical responses of cetuximabtreated cells by immunoblotting. During incubation in serum-free medium, the cells were treated with cetuximab for 24 hours. Cell extracts were freshly prepared and analyzed. Antibo.....
    Document: The CRC cell lines were plated in six-well plates. After 24 hours, the cells were washed with PBS and cultured for 24 hours in serum-free medium. The cell supernatant and protein were collected for immunoblotting. We also detected the biochemical responses of cetuximabtreated cells by immunoblotting. During incubation in serum-free medium, the cells were treated with cetuximab for 24 hours. Cell extracts were freshly prepared and analyzed. Antibodies against EGFR, pAKT (Ser 473 ), AKT, p-p42/44 MAPK (Thr 202 /Tyr 204 ), p42/44 MAPK, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology. The phosphorylation-specific EGFR (Tyr 1068 ) antibody and PRSS1 antibody were obtained from Abcam.

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