Author: Lamborn, Ian T.; Jing, Huie; Zhang, Yu; Drutman, Scott B.; Abbott, Jordan K.; Munir, Shirin; Bade, Sangeeta; Murdock, Heardley M.; Santos, Celia P.; Brock, Linda G.; Masutani, Evan; Fordjour, Emmanuel Y.; McElwee, Joshua J.; Hughes, Jason D.; Nichols, Dave P.; Belkadi, Aziz; Oler, Andrew J.; Happel, Corinne S.; Matthews, Helen F.; Abel, Laurent; Collins, Peter L.; Subbarao, Kanta; Gelfand, Erwin W.; Ciancanelli, Michael J.; Casanova, Jean-Laurent; Su, Helen C.
Title: Recurrent rhinovirus infections in a child with inherited MDA5 deficiency Document date: 2017_7_3
ID: vipx6t7e_56
Snippet: Wild-type IFIH1 (GenBank accession BC111750), or K365E IFIH1 cDNA were subcloned under the human ubiquitin C promoter. VSV-G-pseudotyped lentivirus particles were generated by transient co-transfection into 293T cells of the specific transfer vector with the packaging plasmids pCMV delta R8.2 (HIV-1 GAG/POL, Tat, and Rev expressing plasmid) and pVSV-G (VSV-G envelope expressing plasmid; Stewart et al., 2003) . Cell supernatants were collected dai.....
Document: Wild-type IFIH1 (GenBank accession BC111750), or K365E IFIH1 cDNA were subcloned under the human ubiquitin C promoter. VSV-G-pseudotyped lentivirus particles were generated by transient co-transfection into 293T cells of the specific transfer vector with the packaging plasmids pCMV delta R8.2 (HIV-1 GAG/POL, Tat, and Rev expressing plasmid) and pVSV-G (VSV-G envelope expressing plasmid; Stewart et al., 2003) . Cell supernatants were collected daily for 3 d, filtered through 0.22 µm pore-size filter, concentrated by centrifugation at 18,000× g for 3 h at 4°C, resuspended in Opti-MEM I reduced serum media (Gibco), and stored at −80°C until use.
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