Author: FAZ, Mirna; MARTÍNEZ, José Simón; QUIJANO-HERNÁNDEZ, Israel; FAJARDO, Raúl
Title: Reliability of clinical diagnosis and laboratory testing techniques currently used for identification of canine parvovirus enteritis in clinical settings Document date: 2016_11_6
ID: z3in6bi2_5
Snippet: PCR reactions were performed using 2 µl of each primer (200 nM), 12.5 µl of GoTaq® Green Master Mix (Promega, Madison, WI, U.S.A.) containing DNA polymerase, reaction Buffer (pH 8.5) and 400 µM of each nucleotide (dATP, dGTP, Dctp and dTTP); 3 mM of MgCl 2. and 28.5 µl of nuclease free water. All reactions were carried out under the following amplification conditions; 1 cycle at 94°C for 5 min for initial denaturation, followed by 35 cycles.....
Document: PCR reactions were performed using 2 µl of each primer (200 nM), 12.5 µl of GoTaq® Green Master Mix (Promega, Madison, WI, U.S.A.) containing DNA polymerase, reaction Buffer (pH 8.5) and 400 µM of each nucleotide (dATP, dGTP, Dctp and dTTP); 3 mM of MgCl 2. and 28.5 µl of nuclease free water. All reactions were carried out under the following amplification conditions; 1 cycle at 94°C for 5 min for initial denaturation, followed by 35 cycles at 94°C for 30 sec, 52°C for 1 min, 72°C for 1 min and a final extension cycle at 72°C for 5 min.
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