Research Communications of the 24th ECVIM-CA Congress

Acute intra-alveolar haemorrhage and oedema in the absence of significant inflammatory infiltrates were present in all LPHS lung tissues. Three IHC staining patterns were observed in LPHS lung tissue: alveolar septal wall staining with (IgG n = 8/IgM n = 6) and without intra-alveolar staining (IgG n = 2/IgM n = 0) and staining of intra-alveolar fluid only (IgG n = 1/IgM n = 5). Intra-alveolar staining appeared to favour alveolar surfaces in some cases (IgG n = 5/IgM n = 5). Healthy control lungs showed no staining, whereas haemorrhagic lung showed staining of intraalveolar fluid (IgG/IgM n = 3)) and occasional, mild and discontinuous staining of alveolar septa (n = 1). Leptospiral antigens were not detected in any of the tissues.