Title: Research Communications of the 24th ECVIM-CA Congress Document date: 2015_1_10
ID: r59usk02_186
Snippet: By using the laser microdissection technique, we were able to sample islets that were not contaminated with exocrine tissue, from both healthy and diabetic cats. High RNA quality was confirmed with gel electrophoresis. By quantitative real-time PCR (qRT-PCR), mRNA levels of the islet-specific genes insulin, PDX-1, IAPP, CHGA and IA-2 were detected in both healthy and diabetic cats. We used actin b, GAPDH and RPS7 as internal reference genes for n.....
Document: By using the laser microdissection technique, we were able to sample islets that were not contaminated with exocrine tissue, from both healthy and diabetic cats. High RNA quality was confirmed with gel electrophoresis. By quantitative real-time PCR (qRT-PCR), mRNA levels of the islet-specific genes insulin, PDX-1, IAPP, CHGA and IA-2 were detected in both healthy and diabetic cats. We used actin b, GAPDH and RPS7 as internal reference genes for normalizations of our qRT-PCR data.
Search related documents:
Co phrase search for related documents- gel electrophoresis and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- gel electrophoresis and reference gene: 1, 2
- gel electrophoresis confirm and real time: 1, 2
- gel electrophoresis confirm and reference gene: 1
- gene insulin and mRNA level: 1
- gene insulin and real time: 1
- high RNA quality and real time: 1
- laser microdissection and mRNA level: 1
- laser microdissection and real time: 1, 2, 3
- mRNA level and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- mRNA level and reference gene: 1, 2
Co phrase search for related documents, hyperlinks ordered by date