Title: Research Communications of the 24th ECVIM-CA Congress Document date: 2015_1_10
ID: r59usk02_484
Snippet: Cats that died or were euthanized due to severe illness other than pancreatitis or other pancreatic disease were enrolled. Pancreata were perfused post-mortem with 50 ml Collagenase Type IV (0.5 mg/ml) through the pancreatic duct. The perfused organ was then digested for 30', 40' and 60' at 37°C in a water-bath and purified using a filtration method. Islet cell viability and purity were determined by Thiazolyl Blue Tetrazolium Bromide (MTT assay.....
Document: Cats that died or were euthanized due to severe illness other than pancreatitis or other pancreatic disease were enrolled. Pancreata were perfused post-mortem with 50 ml Collagenase Type IV (0.5 mg/ml) through the pancreatic duct. The perfused organ was then digested for 30', 40' and 60' at 37°C in a water-bath and purified using a filtration method. Islet cell viability and purity were determined by Thiazolyl Blue Tetrazolium Bromide (MTT assay) and dithizone staining, respectively.
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