Author: Neethirajan, Suresh; Ahmed, Syed Rahin; Chand, Rohit; Buozis, John; Nagy, Éva
Title: Recent Advances in Biosensor Development for Foodborne Virus Detection Document date: 2017_7_5
ID: sj6zfybb_119
Snippet: Aptamers are single-stranded oligo nucleotides such as DNA or RNA, or peptides, which bind specifically with high affinity to foodborne viruses [124] . Aptamers can be selected using systematic ligand evolution of ligands by exponential enrichment (SELEX) from a pathogen nucleic acid or peptide library containing at least 10 15 different targeting sequences [125] . A typical SELEX process is to create a random synthesised library of nucleotides, .....
Document: Aptamers are single-stranded oligo nucleotides such as DNA or RNA, or peptides, which bind specifically with high affinity to foodborne viruses [124] . Aptamers can be selected using systematic ligand evolution of ligands by exponential enrichment (SELEX) from a pathogen nucleic acid or peptide library containing at least 10 15 different targeting sequences [125] . A typical SELEX process is to create a random synthesised library of nucleotides, with each nucleotide containing a random central region flanked by different PCR primer binding sequences to facilitate target selection. After selection, the chosen aptamers can recognise and bind to targeting sequences through several properties, such as molecular shape complementarities, hydrogen bonding, electrostatic interactions, or stacking of aromatic rings.
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