Title: Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017 Document date: 2017_11_7
ID: roslkxeq_638
Snippet: All experiments were performed in triplicate. GFP expression was detected by fluorescence microscopy 16 h after infection for all cell lines even with low infective doses. Flow cytometry allowed an assessment of a dose dependent infection of cells. For all cell lines, more than 88% of cells were infected at a MOI of 0.1. Equivalent percentage of infection was noticed for HeLa, 293 and PK-15 at a MOI of 0.01. On the other hand, lower infection was.....
Document: All experiments were performed in triplicate. GFP expression was detected by fluorescence microscopy 16 h after infection for all cell lines even with low infective doses. Flow cytometry allowed an assessment of a dose dependent infection of cells. For all cell lines, more than 88% of cells were infected at a MOI of 0.1. Equivalent percentage of infection was noticed for HeLa, 293 and PK-15 at a MOI of 0.01. On the other hand, lower infection was assessed for DKE1 (55%), A72 (27%) and CrFK (21%). At a MOI of 0.001, higher percentage of infection was observed for 293 (32%) and PK-15 (25%). For the others, less than 13% of cells were infected. Tumoral status of cell lines didn't seem to influence susceptibility to VVTG17990.
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