Selected article for: "lysis buffer and SDS sample buffer"
Author: Ray, Bridgette N.; Kweon, Hye Kyong; Argetsinger, Lawrence S.; Fingar, Diane C.; Andrews, Philip C.; Carter-Su, Christin
Title: Research Resource: Identification of Novel Growth Hormone-Regulated Phosphorylation Sites by Quantitative Phosphoproteomics
  • Document date: 2012_5_8
    • ID: xtj2ywf3_13
    Snippet: 3T3-F442A preadipocytes (15-cm culture dishes) were incubated in serum-free medium overnight before GH treatment. After GH treatment, cells were washed with PBSV, solubilized with 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate supplemented with 1 mM Na 3 VO 4 , 1 mM PMSF, 10 g/ml aprotinin, 10 g/ml leupeptin for 10 min on ice, and then centrifuged for 10 min at 16,000 Ï« g at .....
    Document: 3T3-F442A preadipocytes (15-cm culture dishes) were incubated in serum-free medium overnight before GH treatment. After GH treatment, cells were washed with PBSV, solubilized with 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate supplemented with 1 mM Na 3 VO 4 , 1 mM PMSF, 10 g/ml aprotinin, 10 g/ml leupeptin for 10 min on ice, and then centrifuged for 10 min at 16,000 ϫ g at 4 C. Supernatants were incubated with ␣NHE1 and protein A-agarose beads overnight at 4 C. Beads were washed with lysis buffer, an 80:20 mixture of lysis buffer:SDS-PAGE sample buffer was added, and the samples were boiled for 5 min. Proteins were subjected to SDS-PAGE and immunoblotted as above. Band intensity was quantified using Odyssey software (LI-COR, version 2.0).

    Search related documents:
    Co phrase search for related documents
    • band intensity and ice 10 min: 1
    • band intensity and lysis buffer: 1
    • band intensity and sample buffer: 1
    • culture dish and ice 10 min: 1
    • GH treatment and lysis buffer: 1
    • GH treatment and sample buffer: 1
    • GH treatment and sds PAGE sample buffer: 1
    • GH treatment and serum free medium: 1
    • ice 10 min and lysis buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • ice 10 min and sample buffer: 1, 2, 3, 4, 5
    • ice 10 min and sds PAGE sample buffer: 1, 2
    • ice 10 min and sodium deoxycholate: 1, 2
    • LI COR Odyssey software and lysis buffer: 1, 2
    • LI COR Odyssey software and Odyssey software: 1, 2