Author: Lamborn, Ian T.; Jing, Huie; Zhang, Yu; Drutman, Scott B.; Abbott, Jordan K.; Munir, Shirin; Bade, Sangeeta; Murdock, Heardley M.; Santos, Celia P.; Brock, Linda G.; Masutani, Evan; Fordjour, Emmanuel Y.; McElwee, Joshua J.; Hughes, Jason D.; Nichols, Dave P.; Belkadi, Aziz; Oler, Andrew J.; Happel, Corinne S.; Matthews, Helen F.; Abel, Laurent; Collins, Peter L.; Subbarao, Kanta; Gelfand, Erwin W.; Ciancanelli, Michael J.; Casanova, Jean-Laurent; Su, Helen C.
Title: Recurrent rhinovirus infections in a child with inherited MDA5 deficiency Document date: 2017_7_3
ID: vipx6t7e_32
Snippet: To generate SV40-transformed fibroblast lines, 3 × 10 6 primary dermal fibroblasts were electroporated in 400 µl of complete DMEM with 3 µg of pLAS plasmid (de Chasseval and de Villartay, 1992) in a 0.4-cm cuvette using the Gene Pulser II electroporation system (1 pulse; 250 V, 1,400 µF, resistance = ∞; Bio-Rad Laboratories). Cells were divided into three 75 cm 2 flasks and monitored 1 to 2 wk for outgrowth of rapidly growing colonies. Cult.....
Document: To generate SV40-transformed fibroblast lines, 3 × 10 6 primary dermal fibroblasts were electroporated in 400 µl of complete DMEM with 3 µg of pLAS plasmid (de Chasseval and de Villartay, 1992) in a 0.4-cm cuvette using the Gene Pulser II electroporation system (1 pulse; 250 V, 1,400 µF, resistance = ∞; Bio-Rad Laboratories). Cells were divided into three 75 cm 2 flasks and monitored 1 to 2 wk for outgrowth of rapidly growing colonies. Cultures were then passaged more than four times to ensure elimination of primary fibroblasts. STAT1-deficient SV40-transformed fibroblasts were described previously (Chapgier et al., 2006) .
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