Requirement of CRTC1 coactivator for hepatitis B virus transcription

Tang, Hei-Man Vincent; Gao, Wei-Wei; Chan, Chi-Ping; Cheng, Yun; Chaudhary, Vidyanath; Deng, Jian-Jun; Yuen, Kit-San; Wong, Chun-Ming; Ng, Irene Oi-Lin; Kok, Kin-Hang; Zhou, Jie; Jin, Dong-Yan

preS2-Luc (100 ng), a fixed amount of pcDNA3.1-V5/His-CRTC1/2/3 (100 ng) and escalating amounts of A-CREB plasmid (10 and 20 ng). The differences between groups 2 and 3 (P = 0.0010, highlighted with **) as well as between groups 2 and 4 (P = 0.00099, highlighted with ***) are statistically significant by Student's t test. (C) CREB-mediated preS2 promoter activation was ablated in the presence of dominant inactive CRTC1. HepG2 cells were transfected with preS2-Luc (100 ng), a fixed amount of Tag2B-CREB (100 ng) and escalating amounts of M1-CRTC1 plasmids (25 and 50 ng). The differences between groups 2 and 3 (P = 0.0045, highlighted with **) as well as between groups 2 and 4 (P = 0.00040, highlighted with ***) are statistically significant by Student's t test. (D) CRTC1 recruitment to preS2 promoter was prevented when CREB function was compromised. ChIP was performed as in Figure 2D . The difference between groups 3 and 4 is statistically significant by Student's t test (P = 0.00036, highlighted with ***); N.D.: not detected. Figure S2A and B) . Hence, CRTC1 requires and cooperates with CREB to stimulate HBV transcription.