Selected article for: "gel electrophoresis and Sanger sequencing"
Title: Research Communications of the 24th ECVIM-CA Congress
  • Document date: 2015_1_10
    • ID: r59usk02_190
    Snippet: The objective of this study was to identify the feline AIP gene, identify single nucleotide polymorphisms (SNPs) within this gene and compare any SNPs with reported human AIP SNPs. Stored pituitary tissue from an acromegalic cat was used to create feline AIP cDNA using feline specific AIP primers. Stored EDTA blood from 10 acromegalic cats (diagnosis of insulin resistant diabetes mellitus, serum IGF-1 > 1000 ng/ml and pituitary mass >4 mm identif.....
    Document: The objective of this study was to identify the feline AIP gene, identify single nucleotide polymorphisms (SNPs) within this gene and compare any SNPs with reported human AIP SNPs. Stored pituitary tissue from an acromegalic cat was used to create feline AIP cDNA using feline specific AIP primers. Stored EDTA blood from 10 acromegalic cats (diagnosis of insulin resistant diabetes mellitus, serum IGF-1 > 1000 ng/ml and pituitary mass >4 mm identified using pituitary computed tomography or necropsy) and 10 control cats (no history of diabetes mellitus and greater than 15 years of age) were selected, DNA extracted and genotyped using PCR, agarose gel electrophoresis and Sanger sequencing.

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