Research Communications of the 24th ECVIM-CA Congress

Transcutaneous ICG clearance was performed in 3 healthy research cats and 3 healthy research dogs with normal liver function (bile acid stimulation test, ammonia tolerance test) using a modified device (Kidney Int 2011 79:1254 with an excitation wave length of 760 nm and an emission wave length of 820 nm. The devices were placed on different locations (lateral thoracic wall, ventrolateral abdomen, metatarsus and antebrachium) and fixed with a light bandage. To find a suitable dose to reach adequate transcutaneous peak concentrations escalating doses of ICG (0.1, 0.2 and 0.3 mg/kg) were injected intravenously with a wash out period of at least 24 h in-between. Measurement was continued for 1 hour after injection in awake animals moving freely. The resulting ICG disappearance curves were visually inspected to find best location (minimal artifacts, acceptable background noise) and dose.