Author: FAZ, Mirna; MARTÍNEZ, José Simón; QUIJANO-HERNÁNDEZ, Israel; FAJARDO, Raúl
Title: Reliability of clinical diagnosis and laboratory testing techniques currently used for identification of canine parvovirus enteritis in clinical settings Document date: 2016_11_6
ID: z3in6bi2_6
Snippet: nPCR was initially done amplifying a fragment of 1,740 bp using ParvoExt1f (5′-ATGAGTGATGGAGCAGTTCA-3′) and ParvoExt3R (5′-AGGTGCTAGTTGAGATTTTTCATATAC-3′) primers, and were designed using the nucleotide sequences 1-20 and 1,712-1,740 from gene VP2 for canine parvovirus (GenBank accession number FJ0051962c). Reaction was standardized to a final volume of 50 µl......
Document: nPCR was initially done amplifying a fragment of 1,740 bp using ParvoExt1f (5′-ATGAGTGATGGAGCAGTTCA-3′) and ParvoExt3R (5′-AGGTGCTAGTTGAGATTTTTCATATAC-3′) primers, and were designed using the nucleotide sequences 1-20 and 1,712-1,740 from gene VP2 for canine parvovirus (GenBank accession number FJ0051962c). Reaction was standardized to a final volume of 50 µl.
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