Selected article for: "bind sequence and RNA virus"
Author: Zirkel, Florian; Kurth, Andreas; Quan, Phenix-Lan; Briese, Thomas; Ellerbrok, Heinz; Pauli, Georg; Leendertz, Fabian H.; Lipkin, W. Ian; Ziebuhr, John; Drosten, Christian; Junglen, Sandra
Title: An Insect Nidovirus Emerging from a Primary Tropical Rainforest
  • Document date: 2011_6_14
    • ID: ulwo6i38_27
    Snippet: Genome sequencing. RNA extracted from purified virus preparations was used for unbiased high-throughput sequencing and for conventional sequencing approaches. Genome fragments were generated by adaptorbased random RT-PCR. Following protocols described previously (15, 64) , in this specific study, random hexamers linked to a defined primer sequence tail or oligonucleotides that bind to the conserved TRS elements of CoV linked to an oligonucleotide.....
    Document: Genome sequencing. RNA extracted from purified virus preparations was used for unbiased high-throughput sequencing and for conventional sequencing approaches. Genome fragments were generated by adaptorbased random RT-PCR. Following protocols described previously (15, 64) , in this specific study, random hexamers linked to a defined primer sequence tail or oligonucleotides that bind to the conserved TRS elements of CoV linked to an oligonucleotide anchor were applied (see Table S1 in the supplemental material).

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