Selected article for: "pH Sodium acetate and Sodium acetate"
Author: Ishimaru, Daniella; Plant, Ewan P.; Sims, Amy C.; Yount, Boyd L.; Roth, Braden M.; Eldho, Nadukkudy V.; Pérez-Alvarado, Gabriela C.; Armbruster, David W.; Baric, Ralph S.; Dinman, Jonathan D.; Taylor, Deborah R.; Hennig, Mirko
Title: RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus
  • Document date: 2012_12_26
    • ID: zrbn637z_14
    Snippet: RNA samples were incubated overnight at 37 C to promote dimerization. Samples were crosslinked in a ultraviolet (UV) crosslinker (Spectroline) for 30 min, 254 nm, 1.5 mW/cm 2 on ice (24) . Crosslinked RNA was separated on denaturing PAGE (polyacrylamide gel electrophoresis) and eluted with 0.3 M sodium acetate, 2% sodium dodecyl sulfate (SDS), pH 5.5 at 42 C for 18 h. Precipitated RNA was subjected to partial alkaline hydrolysis. Control, non-cro.....
    Document: RNA samples were incubated overnight at 37 C to promote dimerization. Samples were crosslinked in a ultraviolet (UV) crosslinker (Spectroline) for 30 min, 254 nm, 1.5 mW/cm 2 on ice (24) . Crosslinked RNA was separated on denaturing PAGE (polyacrylamide gel electrophoresis) and eluted with 0.3 M sodium acetate, 2% sodium dodecyl sulfate (SDS), pH 5.5 at 42 C for 18 h. Precipitated RNA was subjected to partial alkaline hydrolysis. Control, non-crosslinked, 32 P-labeled RNA was subjected to RNase T1 digestion or partial alkaline hydrolysis (Applied Biosystems). All samples were separated on denaturing 15% PAGE. Gels were dried and densitometry of bands was determined using ImageQuant TL (GE Healthcare) (25) .

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