Author: Ishimaru, Daniella; Plant, Ewan P.; Sims, Amy C.; Yount, Boyd L.; Roth, Braden M.; Eldho, Nadukkudy V.; Pérez-Alvarado, Gabriela C.; Armbruster, David W.; Baric, Ralph S.; Dinman, Jonathan D.; Taylor, Deborah R.; Hennig, Mirko
Title: RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus Document date: 2012_12_26
ID: zrbn637z_20
Snippet: Total RNA from SARS-CoV and S3L2-ACUucc pk was isolated from infected cell monolayers (Trizol Invitrogen) and purified using Oligotex mRNA spin column reagents (QIAGEN). RNA was separated on an agarose gel using Northern-Max-Gly (Ambion), transfered to a BrightStar-Plus membrane (Ambion) and cross-linked to the membrane with UV light. The blot was pre-hybridized and probed with a SARS-CoV nucleocapsid-specific oligodeoxynucleotide probe (5 0 -CTT.....
Document: Total RNA from SARS-CoV and S3L2-ACUucc pk was isolated from infected cell monolayers (Trizol Invitrogen) and purified using Oligotex mRNA spin column reagents (QIAGEN). RNA was separated on an agarose gel using Northern-Max-Gly (Ambion), transfered to a BrightStar-Plus membrane (Ambion) and cross-linked to the membrane with UV light. The blot was pre-hybridized and probed with a SARS-CoV nucleocapsid-specific oligodeoxynucleotide probe (5 0 -CTTGACTGCCGCCTC
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