RESEARCH COMMUNICATIONS OF THE 28th ECVIM-CA CONGRESS

Formalin‐fixed paraffin embedded samples of canine T‐cell lymphomas, lymphoplasmacellular enteritis, and normal gut tissue were used (n=8 each group). The samples were selected in retrospect from material submitted for diagnostic purposes. Total RNA was extracted and the microRNAs were reverse transcribed. In a pilot study, the expression of 192 different microRNAs was compared on custom‐made arrays for two samples per group. Twelve microRNAs that were highly differentially expressed in the lymphoma group were selected and their expression was compared in all 24 samples using single microRNA qPCR assays. Five of these microRNAs were transfected as microRNA‐mimics and microRNA‐inhibitors into the T‐cell lymphoma cell line CL‐1. Cell viability and cell toxicity were measured after 48h using the Multitox‐Fluor Multiplex Cytotoxicity Assay (Promega).