Title: Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017 Document date: 2017_11_7
ID: roslkxeq_367
Snippet: Urine samples collected from cats presented to the Veterinary Teaching Hospital (VTH, UniTE) or to local veterinary practitioners were screened (Group A, n 60). In addition, urine from cats (Group C, n 72) belonging to 4 colonies were also tested, as well as internal organs of carcasses (Group B, n 35). Formalin-fixed and paraffin embedded sections of kidney of cats were either stained with H&E or incubated with Ab against FeMV-N protein. qPCR-po.....
Document: Urine samples collected from cats presented to the Veterinary Teaching Hospital (VTH, UniTE) or to local veterinary practitioners were screened (Group A, n 60). In addition, urine from cats (Group C, n 72) belonging to 4 colonies were also tested, as well as internal organs of carcasses (Group B, n 35). Formalin-fixed and paraffin embedded sections of kidney of cats were either stained with H&E or incubated with Ab against FeMV-N protein. qPCR-positive RNAs were employed for SISPA/NGS. Finally, a portion of the polymerase (L) gene was amplified by RT-PCR from all positive samples. Carcasses were divided into subgroups, including B1 (+virus +lesions), B2 (+virus -lesions) and B3 (-virus +lesions).
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