RESEARCH COMMUNICATIONS OF THE 28th ECVIM-CA CONGRESS

The selected panel consisted of 7 different microRNAs which have key regulatory role in fibrosis, remodeling and impaired contractility. MiR‐208a, miR‐208b, miR‐499 are located in genes coding different isoforms of myosin heavy chain and are related to impaired contractility. MiR‐133a, miR‐21 and miR‐29 have key role in the regulation of fibrosis and miR‐1 is an important regulator of cardiac hypertrophy. Whole blood and left ventricular samples of tachypaced dogs (n=13), healthy controls (n=7) and whole blood samples (surplus material) of canine clinical patients (n=10) with different severity and etiology of heart failure caused by cardiomyopathy were collected in RNA stabilizing solution. RNA integrity was confirmed by capillary electrophoresis (RIN>7). Expressions of selected microRNAs were measured by qRT‐PCR (TaqMan‐chemistry) and normalized to U6 snRNA by ddCt method. Data evaluation was made by descriptive statistics and Mann Whitney U‐test.