Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017

Disclosures: No disclosures to report. data of serum proteomics on these clinical animals and the modifications in their proteome. Thus, the aim of the present study was to identify the potential serum biomarkers of diagnosis of anaplasmosis and Lyme disease using a proteomic approach. Pools of serum samples from dogs naturally infected with A. phagocytophilum, B. burgdorferi, and infected with both agents were collected before treatment, and compared with a control group. Anaplasmosis and Lyme disease were diagnosed based on specific IgG and IgM antibody titers. Serum C-reactive protein and ferritin levels in infected dogs were higher (P < 0.05) than those of controls. Two-dimensional electrophoresis (2DE) of pooled samples was run in triplicate. 2DE image analysis showed 57 differentially expressed spots between infected animals and controls. Compared to healthy controls, vitamin D-binding protein decreased, while haptoglobin and Ig chains with different spots increased in both diseases. Serum apolipoprotein-A1 (Apo-A1) level decreased in dogs with Lyme, but this did not express differentially in dogs with anaplasmosis. In dual infections, concentrations of vitamin D-binding protein and albumin decreased, whereas alpha glycoprotein, haptoglobin and Ig chain increased compared to controls.