Author: Ma, Ge; Greenwell-Wild, Teresa; Lei, Kejian; Jin, Wenwen; Swisher, Jennifer; Hardegen, Neil; Wild, Carl T.; Wahl, Sharon M.
Title: Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection Document date: 2004_11_15
ID: rlabxfss_5
Snippet: Biotinylation of Recombinant Human SLPI (rhSLPI). rhSLPI (R&D Systems) was biotinylated with EZ-Link Sulfo-NHS-LCbiotin (Pierce Chemical Co.). rhSLPI and NHS-LC-biotin were diluted in PBS on ice and incubated for 2 h. To remove unreacted biotin, the mixture was centrifuged using centricon filters (YM-3; Millipore). After centrifugation, the sample was diluted in PBS, centrifuged twice, and the biotinylated protein concentration was determined (Bi.....
Document: Biotinylation of Recombinant Human SLPI (rhSLPI). rhSLPI (R&D Systems) was biotinylated with EZ-Link Sulfo-NHS-LCbiotin (Pierce Chemical Co.). rhSLPI and NHS-LC-biotin were diluted in PBS on ice and incubated for 2 h. To remove unreacted biotin, the mixture was centrifuged using centricon filters (YM-3; Millipore). After centrifugation, the sample was diluted in PBS, centrifuged twice, and the biotinylated protein concentration was determined (Bio-Rad Laboratories).
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