RESEARCH COMMUNICATIONS OF THE 28th ECVIM-CA CONGRESS

This study aimed to determine the effects of body condition, fat depot, troglitazone, and fatty acids on secretion of adiponectin, interleukin‐6 (IL6), and tumor necrosis factor‐α (TNFα) from adipose tissue of healthy cats. Subcutaneous and visceral adipose tissue samples were collected from 18 healthy intact female cats, and body condition score (BCS, Range 3‐7/9) was determined. Concentrations of adiponectin were measured in mature adipocytes cultures and concentrations of IL6 and TNFα were measured in stromovascular cells (SVC) cultures following treatment with control medium, troglitazone at 10μM, eicosapentaenoic acid (EPA), arachidonic acid (AA), or palmitic acid, at 25μM, 50μM, or 100μM. SVC of visceral origin secreted higher concentrations of IL6 than corresponding cells of subcutaneous origin (P=0.003). AA treatment at 25μM, 50μM, and 100μM increased IL6 secretion in subcutaneous (P=0.045, P=0.002, and P<0.001, respectively) and visceral (P=0.034, P=0.001, and P<0.001, respectively) derived SVC. EPA treatment increased TNFα secretion in subcutaneous derived SVC at 25μM, 50μM, and 100μM (P=0.002, P=0.001, and P=0.015, respectively) and in visceral derived SVC at 50μM (P<0.001). No significant effect on medium adiponectin concentration was observed following troglitazone treatment (P=0.4) or fatty acids treatments at 25μM (P=0.2), 50μM (P=0.8), or 100μM (P=0.7). BCS did not have significant effects on medium concentrations of adiponectin (P=0.4), IL6 (P=0.1), or TNFα (P=0.8). This study demonstrated higher basal secretion of IL6 from visceral compared to subcutaneous adipose tissue, a stimulatory effect of AA on secretion of IL6 and a stimulatory effect of EPA on TNFα from feline adipose tissue.