RESEARCH COMMUNICATIONS OF THE 28th ECVIM-CA CONGRESS

In each BALF, total and differentiated cell counts were obtained and metagenetic analyses were performed on the V1‐V3 hypervariable region of 16S rDNA after total bacterial DNA extraction and sequencing on a MiSeq Illumina sequencer. Taxonomical assignation and microbiota community analysis were done with MOTHUR V1.35 with an OTU clustering distance of 0.03. Statistical comparisons between events for microbiota community and BALF cell counts were made using Friedman test and post‐hoc t‐tests with Bonferroni correction. Statistical differences in bacterial population relative abundance between timepoints were assessed using a mixed linear model with FDR correction for multiple comparisons.