Author: Blank, Maximilian F.; Chen, Sifan; Poetz, Fabian; Schnölzer, Martina; Voit, Renate; Grummt, Ingrid
Title: SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription Document date: 2017_3_17
ID: qm9urt2w_17
Snippet: Cleared cell lysates were incubated for 4 h at 4 • C with the respective antibodies and immunocomplexes were bound to protein G-Sepharose. After washing with buffer containing 200 mM KCl, 0.1% NP-40, protease and HDAC inhibitors, proteins were eluted with the corresponding epitope peptide or with SDS sample buffer and visualized on western blots. In all experiments, controls with unspecific IgGs or no antibody were carried out in parallel......
Document: Cleared cell lysates were incubated for 4 h at 4 • C with the respective antibodies and immunocomplexes were bound to protein G-Sepharose. After washing with buffer containing 200 mM KCl, 0.1% NP-40, protease and HDAC inhibitors, proteins were eluted with the corresponding epitope peptide or with SDS sample buffer and visualized on western blots. In all experiments, controls with unspecific IgGs or no antibody were carried out in parallel.
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