Author: Zhou, Yan; Zheng, HaiHong; Gao, Fei; Tian, DeBin; Yuan, ShiShan
Title: Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA polymerase Document date: 2011_9_16
ID: yo9libo0_16
Snippet: For the analysis of the virus mutational site, a pair of primers, SF8129 and SR10211, was used in the synthesis of cDNA covering the SDD motif at nt 9319-9327. The PCR products were gel-purified and sub-cloned into pGEM-T vector (Promega, Madison, USA), and sequenced......
Document: For the analysis of the virus mutational site, a pair of primers, SF8129 and SR10211, was used in the synthesis of cDNA covering the SDD motif at nt 9319-9327. The PCR products were gel-purified and sub-cloned into pGEM-T vector (Promega, Madison, USA), and sequenced.
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