Author: Liu, Yuan-yuan; Chen, Liang-jun; Zhong, Yan; Shen, Meng-xin; Ma, Nian; Liu, Bing-yu; Luo, Fan; Hou, Wei; Yang, Zhan-qiu; Xiong, Hai-rong
Title: Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo Document date: 2016_3_14
ID: pwlybr2h_12
Snippet: npg complementary oligonucleotides were annealed and inserted into the BamH I/Hind III site of the pSilencer-3.0-H1 vector. After transformation, clone PCR and enzyme digestion, the identified shRNA expression plasmids were sent for sequence analysis for further confirmation and were subsequently designated pSilencer-S and pSilencer-M......
Document: npg complementary oligonucleotides were annealed and inserted into the BamH I/Hind III site of the pSilencer-3.0-H1 vector. After transformation, clone PCR and enzyme digestion, the identified shRNA expression plasmids were sent for sequence analysis for further confirmation and were subsequently designated pSilencer-S and pSilencer-M.
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