Necrotizing Enteritis Caused by Pharyngostomum cordatum Infection in a Stray Cat

Kim, Ji-Hyeon; Lee, Kyunghyun; Sohn, Woon-Mok; Kim, Ha-Young; Lee, Yu-Ran; Choi, Eun-Jin; So, ByungJae; Jung, Ji-Youl

For phylogenetic analysis, sequences were aligned in BioEdit (ver. 7.2.6; Ibis Biosciences, Carlsbad, California, USA) and then analyzed in MEGA7 (Pennsylvania State University, State College, Pennsylvania, USA). Intestinal flukes (Alaria sp., Haplorchis pumilio, Heterophyes heterophyes, Metagonimus yokogawai, and Nanophyetus salmincola), a lung fluke (Paragonimus kellicotti), and a liver fluke (Opisthorchis felineus) were used as outgroups. The sequence in this study (GenBank accession num- (Fig. 4) . The trematode was closely related to the other intestinal flukes, and to the lung and liver fluke. All of these parasites are commonly found in cats. Screening with bacterial isolation and PCR failed to uncover any enterotropic bacterial and viral infections including feline parvovirus and feline coronavirus. To detect viral nucleic acid, commercial PCR/RT-PCR kit were used (iNtRON, Seoul, Korea).